Preparation of Glucosamine Hydrochloride from Crustacean Shell Waste and It's Quantitation by RP-HPLC

Abstract

2 Abstract: Various products derived from crustaceans have food and medicinal values. Glucosamine is an amino monosaccharide acting as substrate for the production of aggrecan and proteoglycans and thus have therapeutic activity in osteoarthritis. The present study has been aimed to prepare glucosamine hydrochloride (Glu-HCl) from various crustacean shells namely Penaeus monodon (Indian shrimp), Portunus pelagicus (blue crab) and Portunus sanguinolentus (three spot crab) by acid hydrolysis and its quantitation by reversed phase high performance liquid chromatography (RP-HPLC). The yield of chitin after demineralization with 0.5M HCl was 87.83%, 89.18% and 51.11% and deacetylation of chitin with 2N NaOH resulted in the yield of 68.91%, 75.67% and 30% for P.sanguinolentus, P.pelagicus and P.monodon respectively. HPLC analysis of obtained glucosamine hydrochloride revealed species of Portunus (21.64 mg g and 21.83 mg g ) were better source 11 of Glu-HCl than P.monodon (3.32 mg g ). Further, this study describes the recycling of crustacean wastes to 1 a value added product which is having potential applications in the field of food and medicine.