Estradiol, Esteriol, Estrone and Novel Flavonoids from Date Palm Pollen

Abstract

Column chromatography of the Egyptian date palm pollen, resulted in the isolation of estradiol, estrone among ten compounds from the hexane fraction. Also, besides rutin, additional four flavonoids from the ethyl acetate extract were isolated. HPLC analysis of the hexane fraction (using RF- BDS C18 - isocratic acetonitrile /water 58:42) revealed the presence of estrone, estradiol and estriol compared with R t of reference standards. The Antioxidant activity by the DPPH method indicated that the ethyl acetate fraction possess a relatively strong antioxidant against DPPH radicals with value (SC50 $ 16.51 µg / ml ). Pollen grains of date palm (Phoenix dactylifera L., 0.5 Kg ) were extracted 3 ×2 L Me OH (each for 2 days) at room temperature. The combined extracts were evaporated to dryness in vacuum to afford 50 g residue. This residue was suspended in 300 ml H2O and then extracted successively with n- hexane, pet ether, CHCl 3 , ether, and EtOAc to yield 5 corresponding fractions (33.7, 0.9, 0.7,, 0.4 and 3 g, respectively).