Production of Transglutaminase by Streptoverticillium ladakanum NRRL-3191 Grown on Media Made from Hydrolysates of Sorghum Straw

Abstract

The aim of this work was to elucidate the suitability of the biotechnological produc-tion of transglutaminase byStreptoverticillium ladakanumNRRL-3191 grown on mediamade from hydrolysates of sorghum straw. Transglutaminase activity was determined infermentations on sorghum straw hydrolysates and commercial xylose with initial xylose10, 20 or 30 g/L. Using media containing commercial xylose 20 g/L, transglutaminase ac-tivity up to 0.282 U/mL was obtained in 96 h. Using neutralized, charcoal-treated hydro-lysates of sorghum straw with xylose 30 g/L sterilized in autoclave at 121 °C, up to 0.155U/mL was obtained in 96 h. However, when the sterilization was performed by filtration,using the same hydrolysates with xylose 20 g/L, up to 0.348 U/mL was obtained in 72 h.It was demonstrated that hydrolysates of sorghum straw are suitable media for transgluta-minase production byStreptoverticillium ladakanum