Identification and Antimicrobial Susceptibility of <em>Salmonella</em> species Isolated from Washing and Rinsed Water of Broilers in Pluck Shops

Abstract

The study was designed with a view to isolate, identifies and characterizes <em>Salmonella</em> species from washing and rinsed water of broilers in pluck shops at Sreepur of Gazipur district in Bangladesh during the period from December 2011 to May 2012. A total of 30 samples collected from the different layers of drums of pluck shops’ were subjected to bacterial isolation and identification by using cultural and biochemical techniques. Furthermore, the isolated <em>Salmonella</em> species were characterized by antimicrobial susceptibility testing. Among the 27 positive <em>Salmonella</em> isolates 11.11% (n = 3) were <em>Salmonella pullorum</em>, 29.83% isolates (n = 8) were <em>Salmonella gallinarum</em> and the rest 59.26% isolates (n = 16) were <em>Salmonella typhimurium</em>. In case of motility test performed by MIU media and hanging drop slide method, 40.74% isolates were non-motile and 59.26% isolates were motile. <em>Salmonella</em> spp. were resistant to doxycyclin and erythromycin. However, most of the <em>Salmonella</em> spp. were susceptible to sulfamethoxazole-trimethoprim and gentamicin. Out of 27 <em>Salmonella isolates</em>, 75% <em>Salmonella typhimurium</em>, 100% <em>Salmonella gallinarum</em> and 100% <em>Salmonella pullorum</em> were detected as multidrug resistant. The findings of the study revealed the presence of multidrug resistant <em>Salmonella</em> species in washing and rinsed water of broilers in Pluck shops at Sreepur of Gazipur district in Bangladesh.