In vitro evaluation of antifungal effects of Lawsonia inermis, Pistacia lentiscus and Juglans regia.

Abstract

The antifungal activity of the hydro alcoholic extracts of Lawsonia inermis, Juglans regia and Pistacia lentiscus, was studied in vitro. Effects of these three plants, largely used in t raditional medicine, were compared with that of Griseofulvine. Trichophyton rubrum, Trichophyton mentagrophyte, Microsporum canisand Candida albicans, 4 stocks dermatophytes and yeast were selected for this study. By the method of diffusion in solid med ium, all the three plants appeared active with concentration of 35mg/ml as compared to control. The hydro alcoholic extract of Pistcia lentiscus was most active by inducing diameters of growth inhibition of 17±1,00 mm and 16,67± 0,58 mm on Trichophyton mentagrophyte and Microsporum canis respectively, except the effect of the 3 extracts on the stock Trichophyton rubrumis comparable. Whereas for the stock Candida albicans, the extract of Lawsonia inermis was most active with a diameter of inhibition of 18,87 ± 0,58mm. By the method of dilution in liquid medium, Pistacia lentiscus and Lawsonia inermis showed antifungal effects on Trichophyton mentagrophytewith one MIC =0,068 mg/ml and on Candida albicans with one MIC =0,034 mg/ml respectively. The study concludes that the hydroalcoholic extracts of L. inermis, J. regia and P. lentiscus possess antifungal activities against the tested strains with variable degrees of sensiti v- ity.