Molecular and Cellular Biology · 2015 · 115 citations · 35 references
Life SciencesCell BiologyMolecular BiologyCell DivisionCell ProliferationMolecular Cell BiologyCell Cycle BiochemistryCell Cycle ControlCell Cycle Gene RegulationCell-cycle RegulationCyclin-dependent KinasesProtein SynthesisCdk1 InactivationCell Cycle-dependent Gene ExpressionCell Division ControlCell Proliferation StimulationCyclin-dependent Kinase 1 ActivationCyclin-dependent Kinase 2 ControlCyclin-dependent Kinase RegulationTranscriptional Regulation By Cyclin-dependent Kinases
The tRNA methytransferase NSun2 promotes cell proliferation, but the molecular mechanism has not been elucidated. Here, we report that NSun2 regulates cyclin-dependent kinase 1 (CDK1) expression in a cell cycle-dependent manner. Knockdown of NSun2 decreased the CDK1 protein level, while overexpression of NSun2 elevated it without altering CDK1 mRNA levels. Further studies revealed that NSun2 methylated CDK1 mRNA in vitro and in cells and that methylation by NSun2 enhanced CDK1 translation. Importantly, NSun2-mediated regulation of CDK1 expression had an impact on the cell division cycle. These results provide new insight into the regulation of CDK1 during the cell division cycle.
35
Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq
Dan Dominissini, Sharon Moshitch-Moshkovitz, Schraga Schwartz et al. · Nature · 2012 · 5K citations