Concepedia

Multiplex PCR Assay Targeting a Diguanylate Cyclase-Encoding Gene, <i>cgcA</i> , To Differentiate Species within the Genus Cronobacter

L. Carter, Lauren Lindsey, Christopher J. Grim, Venugopal Sathyamoorthy, Karen G. Jarvis, Gopal Gopinath, C. Lee, Jennifer Sadowski, Larisa H. Trach, Monica Pava‐Ripoll,

Applied and Environmental Microbiology · 2012 · 57 citations · 16 references

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Concepts

Abstract

In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations.

References

16

MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods

Koichiro Tamura, Daniel G. Peterson, Nora Peterson et al. · Molecular Biology and Evolution · 2011 · 40.3K citations · Full text

, , +17

Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov.

Carol Iversen, Niamh Mullane, B. A. McCardell et al. · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 2008 · 533 citations

, , +15

Cronobacter condimenti sp. nov., isolated from spiced meat, and Cronobacter universalis sp. nov., a species designation for Cronobacter sp. genomospecies 1, recovered from a leg infection, water and food ingredients

Susan Joseph, Esin Çetinkaya, Hana Drahovská et al. · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 2011 · 280 citations

, , +17

Genome Sequence of Cronobacter sakazakii BAA-894 and Comparative Genomic Hybridization Analysis with Other Cronobacter Species

Eva Kucerova, Sandra W. Clifton, Xiao-Qin Xia et al. · PLoS ONE · 2010 · 211 citations · Full text

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Diversity of the Cronobacter Genus as Revealed by Multilocus Sequence Typing

Susan Joseph, Hana Sonbol, S. Hariri et al. · Journal of Clinical Microbiology · 2012 · 174 citations · Full text

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