The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells.

TLDR

The study examined how recombinant BSF‑2/IL‑6 regulates B‑cell growth and differentiation. Recombinant BSF‑2/IL‑6 stimulated Ig production in B‑cell lines and mononuclear cells, enhanced PWM‑induced IgM, IgG, and IgA, but did not promote activated B‑cell growth; it strongly stimulated growth of the MH60.BSF2 hybridoma at 5 pg/ml, and blocking BSF‑2 with antibody inhibited late‑stage Ig production, confirming BSF‑2 as an essential late‑acting factor.

Abstract

The role of recombinant B cell stimulatory factor 2 (BSF-2/IL-6) in the regulation of growth and differentiation of B cells was investigated. rBSF-2 at 200 pg/ml could induce 50% of the maximum Ig production in B lymphoblastoid cell lines, the specific activity being estimated as 5 X 10(6) U/mg. rBSF-2 augmented PWM-induced IgM, IgG, and IgA production in mononuclear cells (MNC); the effect was exerted by directly acting on PWM-induced B blast cells to induce Ig production. However, rBSF-2 did not induce any growth of activated B cells. In contrast, rBSF-2 showed a potent growth activity on a murine hybridoma clone, MH60.BSF2. The concentration required for half-maximal [3H]TdR uptake was approximately 5 pg/ml, which was 40 times less than that required for Ig induction in a B cell line. Anti-BSF-2 antibody inhibited PWM-induced Ig production in MNC, but not PWM-induced proliferation. The antibody was effective even when added on day 4 of an 8-d culture, indicating that BSF-2 is one of the essential late-acting factors in PWM-induced Ig production.