Cross-priming for a secondary cytotoxic response to minor H antigens with H-2 congenic cells which do not cross-react in the cytotoxic assay.

Concepts

Life SciencesImmunologyAdaptive ImmunityComparative ImmunologyVaccine ImmunologyAdaptive Immune ResponsesCell-mediated ImmunityCytotoxic T Lymphocyte ResponsesEffector T Cell FunctionHumoral ImmunityMajor Histocompatibility ComplexAntigen Recognition By Cytotoxic T LymphocytesCytotoxic Effector FunctionMinor Histocompatibility Antigen RecognitionT Cell-mediated Cytotoxicity

TL;DR

The study examines whether H‑2 congenic lymphoid cells express minor histocompatibility determinants that cross‑react at cytotoxic or helper T‑cell levels. Cross‑priming by C57BL/10 and DBA/2 lymphoid cells induces a secondary cytotoxic response in F1 mice that targets B10.D2 and, for DBA/2, also C57BL/10, even though primary cytotoxic assays show no cross‑reactivity.

Abstract

Cytotoxic effector T cells of F1 (BALB/c X BALB.B) (H-2d/b) mice immunized against the minor histocompatibility differences of C57BL/10 (H-2b) can lyse targets from C57BL/10, but cannot lyse B10.D2 (H-2d) targets. Despite this lack of cross-reaction in the cytotoxic assay, C57BL/10 cells do prime F1 (BALB/c X BALB.B) mice for a secondary cytotoxic response to B10.D2. C57BL/10-primed, B10.D2-boosted cytotoxic cells lyse B10.D2 targets but not C57BL/10 targets. DBA/2 (H-2d) spleen cells or thymocytes prime F1 mice for a secondary response to DBA/2, B10.D2, and C57BL/10 cells, but DBA/2 mastocytes, P815, do not prime for a response to C57BL/10. Whether H-2 congenic lymphoid cells express minor histocompatibility determinants which cross-react at the cytotoxic T-cell level or the helper T-cell level is discussed.