Purification and scale-up of a recombinant heavy chain fragment C of botulinum neurotoxin serotype E in Pichia pastoris GS115

Protective vaccination with a recombinant fragment of Clostridium botulinum neurotoxin serotype A expressed from a synthetic gene in Escherichia coli

M A Clayton, Jennifer Clayton, Douglas Brown et al. · Infection and Immunity · 1995 · 149 citations · Full text

Veterinary Medicine, Biotechnology, Immunology +17

Purification, Potency, and Efficacy of the Botulinum Neurotoxin Type A Binding Domain from<i>Pichia pastoris</i>as a Recombinant Vaccine Candidate

Michael Byrne, Theresa J. Smith, Vicki A. Montgomery et al. · Infection and Immunity · 1998 · 126 citations · Full text

Veterinary Medicine, Biotechnology, Immunology +15

Recombinant protein production in an alcohol oxidase-defective strain of Pichia pastoris in fedbatch fermentations

Vijay Chiruvolu, James M. Cregg, Michael M. Meagher · Enzyme and Microbial Technology · 1997 · 122 citations

Life Sciences, Biotechnology, Microbiology +13

Inactivation of <i>Clostridium botulinum</i> type A neurotoxin by trypsin and purification of two tryptic fragments

Clifford C. Shone, Peter Hambleton, J. Melling · European Journal of Biochemistry · 1985 · 122 citations · Full text

Pharmacy, Neuroscience, Toxicology +10

Development of recombinant vaccines for botulinum neurotoxin

Toxicon · 1998 · 103 citations

Veterinary Medicine, Biotechnology, Immunology +14