Molecular assessment of genetic identity and genetic stability in banana cultivars (Musa spp.) from China using ISSR markers.

Abstract

A study was carried out to identify of different cultivars and monitor somaclonal variations of banana during rapid mass micropropgation using Inter simple sequence repeats (ISSR) marker. DNA templates from 30 Banana cultivars were evaluated using 45 primers. Genetic diversity is analyzed by one statistical procedure: hierarchical classification. Total number of bands varied between the various cultivars from 5 9.The percentage of total polymorphism is about 85.1%.Four large group were obtained. Furthermore a homogenous amplification profile was observed for all the micropagated plants of 'Brazil' when compared to their mother plants; the developed profiles of different micropropagated clones were typical to that of the donor mother plants.