Chemical Synthesis of Biologically Active Monoglycosylated GM2‐Activator Protein Analogue Using <i>N</i>‐Sulfanylethylanilide Peptide

Abstract

Going to SEA(lide): Total chemical synthesis of a 162-residue glycoprotein analogue of the monoglycosylated human GM2-activator protein (GM2AP) was achieved. Key steps were the use of N-sulfanylethylanilide (SEAlide) peptides in the kinetic chemical ligation synthesis of a large peptide fragment, and a convergent native chemical ligation for final fragment assembly.