The formation of γ-caseins during cooling of raw milk

Abstract

It has been shown that there is a time-dependent transfer of beta-casein and the milk serine proteinase system from micelles to milk serum with change of temperature from 38 to 4 degrees C. It has been established that the gamma-caseins can be formed by proteolytic degradation of beta-casein. By a simple extraction technique, the very hydrophobic gamma-casein fraction was separated from stored milks (26 and 4 degrees C) and estimated quantitatively. The results showed that the proteolytic degradation of beta-casein is faster at 4 degrees C than at room temperature and this can be explained by the immobilization of enzyme and substrate at the micelle surface at higher temperatures (26 degrees C). The results indicate that irreversible changes during cooling for short periods do not cause problems in milk processing, but the formation of gamma-caseins and phosphopeptides may influence the technological properties of raw milk stored for more than 48 h.