Applied and Environmental Microbiology · 1995 · 77 citations · 13 references
AgricultureFood ScienceMicrobiologyDiagnostic MicrobiologyFood MicrobiologyFood SafetyFoodborne Pathogen DetectionMicrobial Food SafetyMicrobiological Food SafetyMilk MicrobiologyPathogen DetectionPathogen Detection In FoodsDirect DetectionListeria DetectionListeria Monocytogenes In MilkMethod Detection LimitPolymerase Chain Reaction (Pcr) AmplificationPolymerase Chain Reaction (Pcr) AssaysRapid Methods For Food MicrobiologyRaw Milk Microbial Contamination
A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.
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