Improved Resolution of Hydrophobic Penicillin-binding Proteins and Their Covalently Linked Complexes on a Modified C18 Reversed Phase Column

Abstract

ABSTRACT Hydrophobic proteins have proven to be difficult to separate chromatographically. The use of a C18 reversed phase column with embedded polar groups showed improved separation and recovery of the hydrophobic, membrane associated, penicillin-binding proteins of Bacillus subtilis and their covalently linked complexes. The covalently linked complexes were generated by a time course reaction of the non-covalently associated complexes in the intact cell with the paired group specific reagent, cyanogen (C2N2). Keywords:: Hydrophobic membrane proteinsreversed phase chromatographymodified C18 reversed phasepenicillin binding proteinscyanogentime- course aggregationprotein complexes