Angewandte Chemie International Edition · 2005 · 34 citations · 24 references
Life SciencesBiochemistryMolecular BiologyEnzymologyProtein BiochemistryProteomicsActivity-based Protein ProfilingEnzyme Active SitesEnzyme AssaysEnzyme CharacterizationEnzyme ClassificationEnzyme SpecificityActivity-based ScreeningCatalytic ActivityCatalytic Activity AssaysEnzyme Activity AssaysEnzyme FamiliesEnzymesSequence HomologySerine Hydrolases
No longer in a class of its own: By using a gel-free platform for activity-based protein profiling (ABPP; see figure), it was shown that the enzyme sialic acid 9-O-acetylesterase (SAE), which shares no sequence homology with other enzymes, is a member of the serine hydrolase superfamily. The site of fluorophosphonate labeling in SAE was identified as serine 127; this residue is essential for catalytic activity.
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Structural Adaptations in a Membrane Enzyme That Terminates Endocannabinoid Signaling
Michael H. Bracey, Michael A. Hanson, Kim Masuda et al. · Science · 2002 · 488 citations