Isolation and Characterization of the Pigment Esters of Xanthomonas juglandis (campestris)

Abstract

Separation of the pigment esters of Xanthomonas juglandis (campestris) on silica gel columns yielded four bands. Two of the bands, esters 1 and 2, made up over 95% of the mixture. Both esters were phospholipids, with ester 1 containing the alcohol moieties glycerol and sorbitol, and ester 2 only glycerol. Both appeared to be mixtures containing different xanthomonadin pigment molecules but the results suggested that ester 1 consisted of one pigment molecule esterified to glycerophosphoryl sorbitol, whereas ester 2 consisted of 2 pigment molecules esterified to glycerophosphate.