Asp-to-Asn Substitution at the First Position of the DxD TOPRIM Motif of Recombinant Bacterial Topoisomerase I Is Extremely Lethal to E. coli

[6] Use of T7 RNA polymerase to direct expression of cloned genes

F. William Studier, Alan H. Rosenberg, John J. Dunn et al. · Methods in enzymology on CD-ROM/Methods in enzymology · 1990 · 6.6K citations · Full text

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Toprim--a conserved catalytic domain in type IA and II topoisomerases, DnaG-type primases, OLD family nucleases and RecR proteins

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Homogeneous expression of the PBAD promoter in Escherichia coli by constitutive expression of the low-affinity high-capacity AraE transporter

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Interaction between DNA and Escherichia coli protein omega. Formation of a complex between single-stranded DNA and omega protein.

Richard E. Depew, Li‐Fei Liu, J. C. Wang · Journal of Biological Chemistry · 1978 · 169 citations · Full text

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Direct Interaction between Escherichia coli RNA Polymerase and the Zinc Ribbon Domains of DNA Topoisomerase I

Bokun Cheng, Chang-Xi Zhu, Chengling Ji et al. · Journal of Biological Chemistry · 2003 · 112 citations · Full text

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