Concepedia

A Generic High-Throughput Screening Assay for Kinases: Protein Kinase A as an Example

Rommel Mallari, Elissa Swearingen, Wei Liu, Arnold Ow, Stephen W. Young, Shu‐Gui Huang

SLAS DISCOVERY · 2003 · 21 citations · 17 references

Open access

Concepts

PharmacyChemical BiologyMolecular BiologyBiosensorsDrug DesignDrug DiscoveryAssay ValidationBioanalytical Method ValidationEnzyme InhibitionHigh-throughput ScreeningMolecular ScreeningAssayAvidin-biotin Affinity SystemsCoulombic Interactions In ProteinsHigh-throughput Enzyme AssaysHigh-throughput Screening AssaysKinase InhibitorsProtein Kinase InhibitorsScintillation

References

17

A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays

Ji-Hu Zhang, Thomas D.Y. Chung, Kevin R. Oldenburg · SLAS DISCOVERY · 1999 · 7K citations · Full text

Pharmacy, Medicinal Chemistry, Drug Design +16

Protein tyrosine phosphatases in signal transduction

Benjamin G. Neel, N. K. Tonks · Current Opinion in Cell Biology · 1997 · 763 citations

Life Sciences, Biochemistry, Molecular Biology +14

Primary Structural Determinants Essential for Potent Inhibition of cAMP-dependent Protein Kinase by Inhibitory Peptides Corresponding to the Active Portion of the Heat-Stable Inhibitor Protein

David B. Glass, Heung‐Chin Cheng, Liane Mende‐Mueller et al. · Journal of Biological Chemistry · 1989 · 280 citations · Full text

Life Sciences, Biochemistry, Pharmacology +10

Development of High Throughput Screening Assays Using Fluorescence Polarization: Nuclear Receptor-Ligand–Binding and Kinase /Phosphatase Assays

G.J. Parker, Tong Lin Law, Francis J. Lenoch et al. · SLAS DISCOVERY · 2000 · 207 citations · Full text

Life Sciences, Biochemistry, Pharmacology +16

A Microchip-Based Enzyme Assay for Protein Kinase A

Claudia Cohen, Emily Chin-Dixon, Sang Hoon Jeong et al. · Analytical Biochemistry · 1999 · 157 citations

Life Sciences, Biochemistry, Bioengineering +14