Production of Protease from Rice Mill Wastes by Aspergillus niger in Solid State Fermentation

Abstract

Abstract: The production of enzymes by bioprocesses is a good value added to agro industry residues. A comparative study was carried out on the production of protease using different varieties of Rice brokens (PONNI, IR-20, CR-1009, ADT-36 and ADT-66) from Rice mill wastes as substrates in solid-state fermentation (SSF) by Aspergillus niger. Among the all tested varieties of rice broken PONNI produced the highest activity as 67.7 U/g while ADT-66 produced lowest protease as 44.7 U/g / under solid state fermentation conditions. The optimized conditions for producing maximum yield of protease were incubation at 35°C, 96 h and pH 7.0. The protease production from waste treatment could be commercially used in detergents and leather industry. Key words: Solid state fermentation Rice mill wastes Protease