Concepedia

Cloning and Characterization of the Biosynthetic Gene Cluster for Tomaymycin, an SJG-136 Monomeric Analog

Wei Li, Shen‐Chieh Chou, Ankush Khullar, Barbara Gerratana

Applied and Environmental Microbiology · 2009 · 95 citations · 20 references

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Abstract

Tomaymycin produced by Streptomyces achromogenes is a naturally produced pyrrolobenzodiazepine (PBD). The biosynthetic gene cluster for tomaymycin was identified and sequenced. The gene cluster analysis reveals a novel biosynthetic pathway for the anthranilate moiety of PBDs. Gene replacement and chemical complementation studies were used to confirm the proposed biosynthetic pathway.

References

20

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SJG-136 (NSC 694501), a Novel Rationally Designed DNA Minor Groove Interstrand Cross-Linking Agent with Potent and Broad Spectrum Antitumor Activity

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Wei Li, Ankush Khullar, Shen‐Chieh Chou et al. · Applied and Environmental Microbiology · 2009 · 130 citations · Full text

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Phenazine Biosynthesis in <i>Pseudomonas </i><i>f</i><i>luorescens</i>:  Branchpoint from the Primary Shikimate Biosynthetic Pathway and Role of Phenazine-1,6-dicarboxylic Acid

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