Production of Biodiesel with Acid Hydrolysate of<i>Populus Euramevicana CV</i>Leaves by<i>Rhodotorula Glutinis</i>

Abstract

Abstract Populus euramevicana cv leaves acid hydrolysate has been utilized as a substrate for microbial oil production using Rhodotorula glutinis. Uniform design principles and single-factor experiments were employed to investigate the optimal culture conditions of the biodiesel production, which were pH 6.0, inoculum concentration 15%, yeast extract 7 g/L, peptone 3 g/L, hydrolytic saccharide concentration 40 g/L, KH2PO4 0.1%, 50 mL liquid volume in 150 mL shaking flask, and temperature 28°C. Under the optimized conditions, yeast accumulated lipids up to 28.55% on cellular biomass basis with lipid yield 4.62 g/L in 5L bioreactor without detoxification, and R. glutinis accumulated lipids up to 34.16% with lipid productivity reaching 6.18 g/L after detoxification. The analysis of composition of R. glutinis lipid revealed that R. glutinis could be an ideal host for high-quality biodiesel production. The properties of biodiesel produced by this process comply with the standards established by government institute. Keywords: Acid hydrolysateBiodieselPopulus euramevicana cv leavesRhodotorula glutinis ACKNOWLEDGMENTS The authors are thankful to the high technology project (BG2005326) of the Council of Science and Technology of JiangSu Province (China) for providing financial assistance.