Detection of total and pathogenic Vibrio parahaemolyticus by Polymerase chain reaction using toxR, tdh and trh genes

Abstract

The levels of total and pathogenic Vibrio parahaemolyticus were estimated in 105 samples (each 35 of fresh water fish, sea fish and mutton) using cultural and polymerase chain reaction (PCR) methods by amplification of toxR gene for total V. parahaemolyticus, tdh and trh genes for pathogenic V. parahaemolyticus. Out of 105 samples, 30 (28.6%), 39 (37.1%) samples gave positive results for total V. parahaemolyticus by cultural and PCR methods respectively. Out of 39 samples positive for PCR 6 (5.7%), 28 (26.6%) and 3 (8.6%) were positive for tdh, trh and both tdh and trh respectively.