Isolation of Aureobasidium pullulans from bathroom surfaces and their antifungal activity against some Aspergilli

Abstract

Ten Aureobasidium isolates were collected from bathroom surfaces in Thailand.  They were identified as Aureobasidium pullulans.  Cell extracts from all isolates were tested for antifungal activities against four selected Aspergillus species using a paper disc diffusion and conidial germination inhibition assay.  BM1, KT1, HKW1 and HKW2 extracts inhibited Aspergillus terreus, whereas KT1 and BM1 extracts also inhibited Aspergillus fumigatus.  BM1 extract alone inhibited Aspergillus flavus.  From TLC analysis, an antifungal compound with an identical Rf to that of aureobasidin A was found in all extracts.  Antifungal tests of TLC-separated compounds supported the paper disc diffusion and conidial germination inhibition assays.    Key words: Aureobasidium pullulans, antifungal activity, aureobasidin.