Antimicrobial potential and bioactive constituents from aerial parts of Vitis setosa Wall.

Abstract

Plants still represent untapped sources of novel compounds with potential therapeutic effects. Hence, the present investigation was carried out to study the in vitro evaluation of antimicrobial potential and to assess the bioactive constituents of the aerial parts of Vitis setosa Wall. The powdered aerial material was extracted with different solvents and examined for antimicrobial activity against Escherichia coli, Salmonella typhi, Bacillus subtilis, Streptococcus pyogenes, Candida albicans, Fusarium solani and Trichophyton rubrum using well diffusion method. An ethanol extract of V. setosa showed significant antimicrobial activity against all the tested pathogens. The fresh aerial parts contained eight primary metabolites (chlorophylls, carotenoids, total soluble sugars, total soluble proteins, total free amino acids, total phenol, hydroxyl phenols and lipids) and alkaloids, tannins, saponins, triterpenoids and phenolic compounds. Gas chromatography-mass spectrometry (GC-MS) results also showed 26 bioactive compounds including n-hexadecanoic acid, 9,12,15-octadecatrienoic acid (z,z,z)- and τ-tocopherol. In conclusion, the aerial parts of V. setosa are a promising source of antimicrobial bioactive compounds.   Key words: Bioactive constituents, Vitis setosa, GC-MS analysis, ethanolic extract, antimicrobial activity, folk medicine.