Transcription Analysis of Class II Human Leukocyte Antigen Genes from Normal and Immunodeficient Β Lymphocytes, Using Polymerase Chain Reaction

Abstract

The RNA transcript levels of all human leukocyte antigen class II loci were determined from class II congenital immunodeficient B cells by polymerase chain reaction amplification of cDNA. No mRNA was observed under conditions in which 0.01% normal levels could be visualized. Pre-mRNA could be amplified from normal B cells but not from immunodeficient B cells, indicating a transcription defect.