Concepedia

Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA

Phyllis Ponte, S Y Ng, Joanne N. Engel, Peter W. Gunning, Larry Kedes

Nucleic Acids Research · 1984 · 965 citations · 14 references

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Abstract

We report the complete nucleotide sequence of a human beta actin cDNA. Both the 5' and 3' untranslated regions of the sequence are similar (greater than 80%) to the analogous regions of the rat beta-actin gene reported by Nudel et al (1983). When a segment of the 3' untranslated region is used as a radiolabelled probe, strong hybridization to chick beta actin mRNA is seen. This conservation of sequences suggests that strong selective pressures operate on non-translated segments of beta actin mRNA.

References

14

3′ Non-coding region sequences in eukaryotic messenger RNA

Nicholas Proudfoot, G.G. Brownlee · Nature · 1976 · 3K citations

, , +14

Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probes

Don W. Cleveland, Margaret A. Lopata, Raymond J. MacDonald et al. · Cell · 1980 · 1.9K citations

, , +10

Isolation and Characterization of Full-Length cDNA Clones for Human α-, β-, and γ-Actin mRNAs: Skeletal but Not Cytoplasmic Actins Have an Amino-Terminal Cysteine that Is Subsequently Removed

Peter W. Gunning, Phyllis Ponte, Hiroto Okayama et al. · Molecular and Cellular Biology · 1983 · 1.2K citations · Full text

, , +8

The nucleotide sequence of the rat cytoplasmic β–actin gene

Uri Nudel, Rina Zakut, Moshe Shani et al. · Nucleic Acids Research · 1983 · 1K citations · Full text

, , +8

A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.

Hiroto Okayama, P Berg · Molecular and Cellular Biology · 1983 · 935 citations

, , +15