Synthesis and Hybridization Properties of Sugar-Modified Oligonucleotides

Abstract

L-Threoninol-derived acyclic nucleotide monomers were prepared and incorporated into oligonucleotides at preselected positions via phosphoramidite chemistry. Hybridization properties of these modified oligonucleotides with the corresponding natural oligomers were studied, and their vis-à-vis comparison with serinol-modified oligonucleotides was made. Stability of the modified oligomers against nuclease in human serum and snake venom phosphodiesterase (SVPD) was examined.