MULTISPECTRAL DETECTION OF FECAL CONTAMINATION ON APPLES BASED ON HYPERSPECTRAL IMAGERY: PART I. APPLICATION OF VISIBLE AND NEARINFRARED REFLECTANCE IMAGING

Abstract

Fecal contamination of apples is an important food safety issue. To develop automated methods to detect suchcontamination, a recently developed hyperspectral imaging system with a range of 450 to 851 nm was used to examinereflectance images of experimentally contaminated apples. Fresh feces from dairy cows were applied simultaneously as athick patch and as a thin, transparent (not readily visible to the human eye), smear to four cultivars of apples (Red Delicious,Gala, Fuji, and Golden Delicious). To address differences in coloration due to environmental growth conditions, apples wereselected to represent the range of green to red colorations. Hyperspectral images of the apples and fecal contamination siteswere evaluated using principal component analysis with the goal of identifying two to four wavelengths that could potentiallybe used in an online multispectral imaging system. Results indicate that contamination could be identified using either threewavelengths in the green, red, and NIR regions, or using two wavelengths at the extremes of the NIR region underinvestigation. The three wavelengths in the visible and nearinfrared regions offer the advantage that the acquired imagescould also be used commercially for color sorting. However, detection using the two NIR wavelengths was found to be lesssensitive to variations in apple coloration. For both sets of wavelengths, thick contamination could easily be detected usinga simple threshold unique to each cultivar. In contrast, results suggest that more computationally complex analyses, such ascombining threshold detection with morphological filtering, would be necessary to detect thin contamination spots usingreflectance imaging techniques.