An efficient 1-tube PCR method for internal site-directed mutagenesis of large amplified molecules

Abstract

Journal Article An efficient 1-tube PCR method for internal site-directed mutagenesis of large amplified molecules Get access Frank Marini, III, Frank Marini, III Department of Molecular Pathology, The University of Texas M.D.Anderson Cancer Center1515 Holcombe Boulevard, Houston, TX 77030, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Amyr Naeem, Amyr Naeem Department of Molecular Pathology, The University of Texas M.D.Anderson Cancer Center1515 Holcombe Boulevard, Houston, TX 77030, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Jean-Numa Lapeyre Jean-Numa Lapeyre * Department of Molecular Pathology, The University of Texas M.D.Anderson Cancer Center1515 Holcombe Boulevard, Houston, TX 77030, USA * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Nucleic Acids Research, Volume 21, Issue 9, 11 May 1993, Pages 2277–2278, https://doi.org/10.1093/nar/21.9.2277 Published: 11 May 1993 Article history Received: 06 January 1993 Revision received: 24 March 1993 Accepted: 24 March 1993 Published: 11 May 1993