Gene · 1990 · 640 citations · 13 references
Life SciencesBiotechnologyMolecular BiologyGenetic EngineeringMolecular BiotechnologyMolecular Cloning WorkflowsMutagenesis StrategiesPolymerase Chain ReactionSite-directed MutagenesisDirected MutagenesisPcr-based Mutagenesis
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Site-directed mutagenesis by overlap extension using the polymerase chain reaction
Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations
Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA
Ellson Y. Chen, Peter H. Seeburg · DNA · 1985 · 2.5K citations