Bioscience Biotechnology and Biochemistry · 2005 · 40 citations · 25 references
Life SciencesFood ScienceMolecular BiologyAllergyFood MicrobiologyFood SafetyAllergy DiagnosticsFood AllergensFood AllergyFood Safety AssessmentMolecular DiagnosisPolymerase Chain Reaction16S-23s Internal Transcribed Spacer AnalysisAllergen AuthenticationFood Allergen ResponsesFood Allergen SourcesInternal Transcribed Spacer SequencingMajor Food AllergensMolecular Allergy DiagnosticsSevere Allergic Reaction
Buckwheat often causes severe allergic reactions, even when its ingestion level is extremely low. Therefore, buckwheat is listed in several countries as a common food allergen. In addition to common buckwheat and Tartarian buckwheat that are cultivated and consumed widely, wild buckwheat may be potentially allergenic. Food containing undeclared buckwheat poses a risk to patients with the buckwheat allergy. We describe in this report a PCR method to detect buckwheat DNA by using primers corresponding to the internal transcribed spacer region and the 5.8S rRNA gene. The method is buckwheat-specific and compatible with both cultivated and wild buckwheat of the Fagopyrum spp. Its sensitivity was sufficient to detect 1 ppm (w/w) of buckwheat DNA spiked in wheat DNA. This method should benefit food manufacturers, clinical doctors, and allergic patients by providing information on the presence of buckwheat contamination in food.
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Universal primers for amplification of three non-coding regions of chloroplast DNA
Pierre Taberlet, Ludovic Gielly, Guy Pautou et al. · Plant Molecular Biology · 1991 · 5.7K citations