Analysis of complex lipid mixtures by thin‐layer chromatography and complementary methods

Abstract

Conclusion Further work has been done on the application of thin‐layer adsorption chromatography to the fractionation of complex lipid mixtures into classes. New methods, the use of siliconized silicic acid plates and the application of thin‐layer adsorption chromatography combined with the complementary techniques of gas‐liquid chromatography and paper chromatography, are presented for the resolution of classes of lipids into their constituents. In contrast to such elaborate conventional techniques as column chromatography, analyses using the methods reported in this paper can be performed rapidly in large numbers on a routine basis.