Extraction, characterization and in vitro antioxidative potential of chitosan and sulfated chitosan from Cuttlebone of Sepia aculeata Orbigny, 1848

Abstract

To investigate antioxidant potency of chitosan and sulfated chitosan in various established in vitro systems, such as superoxide (O2-)/hydroxyl (-OH) radicals scavenging, reducing power, metal ion chelating. Chitosan was prepared from deacetylation of chitin from cuttlefish. FTIR, degree of acetylation and mineral studies was carried out from the chitosan. Chitosan was converted into sulfated chitosan using DMF/HClSO3 and their antioxidant activity was tested. Mineral content of chitosan was Ca – 30 ppm, Na- 0.092 ppm, Cu-0.172 ppm, Mg- 3.601, Mn- 0.264 and Zn- 0.924 ppm, DA was 49.9% (IR spectroscopy) and 40.56% (UV spectrophotometer) recorded. Cuttlefish chitosan showed high level of superoxide radical scavenging activity (88.6%) and hydroxyl radical scavenging activity (72.1%), moderate activity was noted in chelating activity (62.6% at 100 μgm/mL). At the same time low level of reducing power was observed (0.300 Absorbance at 0.75 mg/mL) when compared to standard BHA and Ascorbic acid (2.305 and 2.05 Absorbance). Finally, the scavenging rate, reducing power, chelating and reducing power of sulfated chitosan increased with their increasing concentration.