Detection of Irradiated Wheat Using the Electron Paramagnetic Resonance Spin Probe Technique

Abstract

ABSTRACT In this study, the electron paramagnetic resonance (EPR) spin probe technique is applied as a detection method in the differentiation of irradiated and unirradiated wheat seeds. Two wheat cultivars, Kunduru and Bezostaya, were used. Aqueous solutions of 4‐hydroxy‐TEMPO (TANOL) spin probe prepared with a line‐broadening agent, potassium ferricyanide, were used in all experiments. The EPR spectra of the samples were recorded against time. A decrease in the signal intensity and a change in the shape of the intensity‐time curve (rehydration curve) were observed, depending on the applied level of irradiation. The ratio of the lipid and aqueous regions at the high field (m I = ‐1) line changes, depending on the dose of irradiation.