Development of an efficient GLC system with a mixed chiral stationary phase and its application to the separation of optical isomers

Abstract

Abstract In order to overcome the limitations of the methodology for the separation of enantiomers in GLC analysis, we successfully developed a novel GLC column named ‘Chiramix’, coated with a mixture of two chiral stationary phases, heptakis‐(2,6‐di‐ O ‐methyl‐3‐ O ‐pentyl)‐β‐cyclodextrin (2,6‐Me‐3‐Pe‐β‐CD) and octakis‐(2,6‐di‐ O ‐methyl‐3‐ O ‐trifluoroacetyl)‐γ‐cyclodextrin (2,6‐Me‐3‐TFAc‐γ‐CD). It was proved that the ‘Chiramix’ column is greatly superior to those with a single chiral phase for separating enantiomers of compounds with various functional groups. As a successful example, this was applied to the analysis of multicomponent systems, such as peach flavours, and the enantiomeric purities of several components of monoterpene hydrocarbons, alcohols, ketones and lactones were determined exactly and simultaneously. Copyright © 2001 John Wiley & Sons, Ltd.