ANTIOXIDANT ACTIVITY AND PHENOLIC ACID CONSTITUENTS OF CHESTNUT (<i>CASTANIA SATIVA</i>MILL.) HONEY AND PROPOLIS

Abstract

ABSTRACT This study describes the constituents of phenolic acids and antioxidant activities of chestnut (Castania sativa Mill.) honeys and propolis in Turkey. Antioxidant activity of the chestnut honeys and propolis were examined by three different methods, namely scavenging of free radical 2, 2-diphenyl-1-picrylhydrazyl, FRAP, and cupric reducing antioxidant power. Total phenolic contents were determined by using Folin–Ciocalteu reagent as GA equivalent. The phenolic constituents were also determined by HPLC. The antioxidant activities were compared with standard antioxidants such as catechin, BHT and Trolox. The antioxidant activities of all the samples were found high and related to the sample concentrations. The ethanolic propolis extracts showed the highest antioxidant activity. The major phenolic acids of the chestnut honeys and propolis identified by HPLC with PDA detection were coumaric acid, FA, cinnamic acid, CA and ChA. PRACTICAL APPLICATIONS In this study, some phenolic acid components and antioxidant capacity of chestnut (Castania sativa Mill.) honey and propolis were measured. The comparative findings from antioxidant activities and phenolic acid analyses of honey and propolis samples of chestnut origin provide important criteria for considering their nutritional and nutraceutical potentials. Comparison of our results with literature data also ranks the chestnut honey and propolis as better sources of antioxidants among those from other floral origins.