Dynamic Kinetic Resolution Process Employing Haloalkane Dehalogenase

Abstract

The first dynamic kinetic resolution process with a haloalkane dehalogenase is described, allowing the efficient preparation of enantiopure α-hydroxyamides from racemic α-bromoamides. A simple membrane reactor is used to separate the enzyme from the nonsoluble, polymer-based, and metal-free racemizing agent. A model substrate, N -phenyl-2-bromopropionamide, was converted to ( S )- N -phenyl-2-hydroxypropionamide either with 63% yield and 95% e.e or with 78% yield and 88% e.e.