Concepedia

The ClosTron: Mutagenesis in Clostridium refined and streamlined

John Heap, Sarah A. Kuehne, Muhammad Ehsaan, Stephen T. Cartman, Clare Cooksley, Jamie Scott, Nigel P. Minton

Journal of Microbiological Methods · 2009 · 419 citations · 27 references

DOI

Concepts

References

27

Site-directed mutagenesis by overlap extension using the polymerase chain reaction

Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations

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Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant

Peter Cherepanov, Wilfried Wackernagel · Gene · 1995 · 2K citations

, , +17

Bile Salts and Glycine as Cogerminants for <i>Clostridium difficile</i> Spores

Joseph A. Sorg, Abraham L. Sonenshein · Journal of Bacteriology · 2008 · 724 citations · Full text

, , +15

The ClosTron: A universal gene knock-out system for the genus Clostridium

John Heap, Oliver Pennington, Stephen T. Cartman et al. · Journal of Microbiological Methods · 2007 · 671 citations

, , +5

A modular system for Clostridium shuttle plasmids

John Heap, Oliver Pennington, Stephen T. Cartman et al. · Journal of Microbiological Methods · 2009 · 503 citations

, , +13