Korean Journal of Clinical Microbiology · 2009 · 16 citations · 8 references
Life SciencesInfectious DiseasesMicrobiologyBacteriologyMedical MicrobiologyMicrobial PathogenesisBacterial EpidemiologyBacterial PathogenesisBacterial ToxinsFoodborne PathogensMicrobial ContaminationToxin ProductionBacillus Cereus ToxinsBacterial ExotoxinsBacterial Toxin AssaysGel ElectrophoresisPathogenesis Of Bacterial ToxinsPulsed-field Gel ElectrophoresisPulsed-field Gel Electrophoresis TypingToxin-producing Bacteria
Bacillus cereus causes two types of gastrointestinal diseases: emesis and diarrhea. It produces one emetic toxin and nine different enterotoxins. In March 2008, eight of a family became sick after eating slices of raw fish. We isolated emetic toxin producing B. cereus from the stools of 6 patients and 2 sub-clincal humans. In this study, the presence of enterotoxin genes, such as those of haemolysin BL (Hbl), nonhemolytic enterotoxin (Nhe), B. cereus enterotoxin T (BceT), enterotoxin FM (EntFM), cytotoxin K (cytK) and cereulide were assayed by polymerase chain reaction (PCR) methods. Their enterotoxin activities were assayed using the BCET- RPLA, Tecra ELISA kit and Hep-2 vacuole activity. Bacterial isolates were subtyped by pulsed-field gel electrophoresis (PFGE). This study demonstrates the emetic toxin-producing stains of B. cereus in clinical specimens, for the first time in the Republic of Korea.
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Bacillus cereus and other Bacillus species.
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Life Sciences, Biology, Microbiology +10
A novel dodecadepsipeptide, cereulide, is an emetic toxin of
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Monika Ehling‐Schulz, Nataša Vukov, Anja Schulz et al. · Applied and Environmental Microbiology · 2005 · 286 citations · Full text