High pressure liquid chromatographic separation of molecular species of phosphatidic acid dimethyl esters derived from phosphatidylcholine

Abstract

Abstract A majority of the individual molecular species of phosphatidic acid dimethyl esters derived from multispecies egg yolk and soybean phosphatidylcholines have been separated by reverse‐phase high pressure liquid chromatography. Two Partisil‐10 ODS columns connected in tandem and the eluents acetonitrile or methanol/water (95∶5) were used for molecular species resolution, based on total fatty acyl carbon number and degree of unsaturation.