Labile exocyclic amine protection of nucleosides in DNA, RNA and oligonucleotide analog synthesis facililating N-deacylation, minimizing depurination and chain degradation

Polymer support oligonucleotide synthesis XVIII^1.2): use of<i>β</i>-cyanoethyi-N,N-dialkylamino-/N-morpholino phosphoramidite of deoxynucleosides for the synthesis of DNA fragments simplifying deprotection and isolation of the final product

N. D. SINHA, J. Biernat, J. F. A. McManus et al. · Nucleic Acids Research · 1984 · 848 citations · Full text

Pharmacy, Biotechnology, Molecular Biology +16

Transient protection: efficient one-flask syntheses of protected deoxynucleosides

Gavrilova Ti, Barbara L. Gaffney, Roger A. Jones · Journal of the American Chemical Society · 1982 · 389 citations

Pharmacy, Chemistry, Analytical Chemistry +4

The automated chemical synthesis of long oligoribuncleotides using 2'-O-silylated ribonucleoside 3'-O-phosphoramidites on a controlled-pore glass support: synthesis of a 43-nucleotide sequence similar to the 3'-half molecule of an Escherichia coli formylmethionine tRNA

Nassim Usman, Kelvin K. Ogilvie, Mingzhu Jiang et al. · Journal of the American Chemical Society · 1987 · 367 citations

Pharmacy, Molecular Biology, Bioorthogonal Chemistry +11

The automated synthesis of sulfur-containing oligodeoxyribonucleotides using 3H-1,2-benzodithiol-3-one 1,1-dioxide as a sulfur-transfer reagent

Radhakrishnan P. Iyer, Lawrence R. Phillips, William Egan et al. · The Journal of Organic Chemistry · 1990 · 230 citations

Natural Sciences, Biochemistry, Chemistry +12

The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups

J.C. Schulhof, D. Molko, R. Téoule · Nucleic Acids Research · 1987 · 199 citations · Full text

Pharmacy, Biochemistry, Molecular Biology +13