LIPASE ACTIVITY AND STABILITY IN SUPERCRITICAL CARBON DIOXIDE

Abstract

Abstract The use of a supercritical fluid as a medium for enzymatic reaction was investigated with respect to its application and reaction mechanism. Lipase could catalyze the reaction of hydrolysis and interes-terification in supercritical carbon dioxide at 35°C and 137 bar. There was no difference in the time course of interesterification between the untreated lipase and the lipase exposed to supercritical carbon dioxide indicating that the enzyme was not severely damaged.