Tagging a T. brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects

A tightly regulated inducible expression system for conditional gene knock-outs and dominant-negative genetics in Trypanosoma brucei

Elizabeth Wirtz, Simone Leal, Claudia M. Ochatt et al. · Molecular and Biochemical Parasitology · 1999 · 1.5K citations

Life Sciences, Genetics, Molecular Genetics +17

Continuous Cultivation of Trypanosoma brucei Blood Stream Forms in a Medium Containing a Low Concentration of Serum Protein without Feeder Cell Layers

H. Hirumi, K. Hirumi · Journal of Parasitology · 1989 · 1K citations · Full text

Veterinary Medicine, Infectious Diseases, Microbiology +13

Double-stranded RNA induces mRNA degradation in <i>Trypanosoma</i> <i>brucei</i>

Huân M. Ngô, Christian Tschudi, Keith Gull et al. · Proceedings of the National Academy of Sciences · 1998 · 715 citations · Full text

Life Sciences, Genetics, Molecular Biology +16

Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system.

Wolfram Saenger, Winfried Hinrichs, Peter Orth et al. · Nature Structural Biology · 2000 · 473 citations

Life Sciences, Genetics, Molecular Biology +16

Improvements in transfection efficiency and tests of RNA interference (RNAi) approaches in the protozoan parasite Leishmania

Kelly A. Robinson, Stephen M. Beverley · Molecular and Biochemical Parasitology · 2003 · 284 citations

Life Sciences, Biology, Molecular Biology +14