Identification of barley and wheat cDNA clones related to the high‐<i>M</i>_r polypeptides of wheat gluten

Abstract

We have identified 3 cDNA clones related to the high‐ M r group of storage proteins in barley endosperm, the D‐hordeins. A cDNA library has been constructed from wheat endosperm poly(A + )‐RNA and screened using one of the D‐hordein cDNA clones. Two wheat clones which cross‐hybridised to the barley clone have been identified, by hybrid‐release translation and nucleotide sequence analysis, as partial copies of mRNAs encoding the high‐ M r gluten polypeptides of wheat.