Synthesis, Structural and Biological Studies of Ubiquitin Mutants Containing (2<i>S</i>, 4<i>S</i>)‐5‐Fluoroleucine Residues Strategically Placed in the Hydrophobic Core

Abstract

Insider report: A fluoroleucine mutant of ubiquitin (cyan) with two strategically placed fluorine reporter groups in its core was synthesised. 1H NMR, crystallography, CD spectroscopy, biological assay and calorimetry indicate minimal structural and functional perturbation compared to the native protein (yellow; see picture). 19F NMR spectra indicate changes within the core during folding and unfolding.