Science · 1992 · 215 citations · 31 references
Life SciencesImmunologyMolecular BiologyAutoimmunityImmunogeneticsProtein-protein InteractionsAntigen PresentationAntigen ProcessingHistocompatibility AntigensHuman Leukocyte Antigen SystemMajor Histocompatibility ComplexProtein SynthesisAberrant Antigen ProcessingAntigen Processing DefectsClass Ii Histocompatibility AntigensEndosomal ProcessingHla Class Ii MoleculesInvariant Chain ProcessingMajor Histocompatibility Complex Class Ii Antigen ProcessingMhc Class Ii Molecules
Class II major histocompatibility complexes bind peptides in an endosome-like compartment. When the class II null cell line 721.174 was transfected with class II DR3 genes, DR molecules were produced in normal amounts. However, the DR molecules were abnormally conformed and unstable because deletion of an antigen-processing gene had impaired intracellular formation of most class II-peptide complexes. Yet, 70 percent of the DR molecules still bore peptides, 80 percent of which were 21- to 24-amino acid fragments of the class II-associated invariant chain. These peptides were rare on DR3 from control cells. Thus, a defect in the main antigen-processing pathway revealed a process in which DR molecules bind long peptides derived from proteins present in the same compartment.
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Sequence analysis of peptides bound to MHC class II molecules
Alexander Y. Rudensky, Paula Preston‐Hurlburt, Soon–Cheol Hong et al. · Nature · 1991 · 1.1K citations
Specificity pockets for the side chains of peptide antigens in HLA-Aw68
Thomas Garrett, Mark A. Saper, Pamela J. Björkman et al. · Nature · 1989 · 657 citations