Journal of Eukaryotic Microbiology · 2007 · 12 citations · 13 references
Natural SciencesChemistryMicrobiologyAnalytical ChemistryMass Spectrometric AnalysisSpectroscopyMass AnalyzersMass SpectrometryMass Spectrometry Data AnalysisOocyst DetectionCryptosporidium Oocyst DetectionMaldi-tof Mass SpectrometryMass Spectrometric IdentificationMass Spectrometry CharacterizationMatrix-assisted Laser Desorption Ionization Time-of-flight Mass SpectrometryMatrix-assisted Laser Desorption/ionizationMatrix-assisted Laser Desorption/ionization Time-of-flight Mass SpectrometryTime-of-flight Mass Spectrometry
Cryptosporidium parvum oocysts were analyzed using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Sample preparation proved to be a crucial step in the acquisition of acceptable mass spectra. Oocysts of C. parvum and the matrix were mixed and held for at least 45 min to produce reproducible, representative mass spectra. Sporozoites were also excysted from oocysts, purified, and analyzed using MALDI-TOF MS. The mass spectra of the intact oocysts contained many of the same peaks found in the mass spectra of the sporozoites, suggesting that during analysis, the internal constituents, not just the oocyst wall, are ablated by the laser.
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Tracie L. Williams, Denis Andrzejewski, Jackson O. Lay et al. · Journal of the American Society for Mass Spectrometry · 2003 · 210 citations
Michael J. Arrowood, Kimberley B. J. Donaldson · Journal of Eukaryotic Microbiology · 1996 · 186 citations