Biotechnology and Bioengineering · 1971 · 11 citations · 9 references
Life SciencesBioengineeringBiotechnologyCell BiophysicsCell PhysiologyCellular EngineeringBatch CultureCell CultureCell Culture TechnologyCell GrowthCell HarvestingContinuous CultureCell Culture ContaminationContinuous Cell HarvestingContinuous Culture GrowthPh Control In CultureSuspension Cell CultureSuspension Culture
Abstract A 30‐liter apparatus for growing cells continuously is described. Samples are taken automatically, thus obviating one of the main causes of contamination. In order to eliminate the continuous introduction of a gas stream, the pH was kept constant by means of the injection of NH 4 OH or NaOH. Under these circumstances, and by means of the two methods of agitation described—rotary and “by Vibromixer”—a quantity of cells of approximately 4 × 10 10 is harvested every 48 to 72 hr.
9
The Submerged Culture of Mammalian Cells: The Spinner Culture,
William F. McLimans, Eldon V. Davis, Fletcher L. Glover et al. · The Journal of Immunology · 1957 · 361 citations