Biotechnology and Bioengineering · 1965 · 81 citations · 7 references
EngineeringBioengineeringBiotechnologyCell EngineeringCellular EngineeringIndustrial BiotechnologyAnimal Cell CultureCell CultureCell Culture BioreactorsCell Culture TechnologyContinuous CultureMammalian Cell CultureBioreactor Culture SystemsCell Culture BioprocessingCell Culture ConditionsCell Culture PerformanceCell Culture Process DesignPh DependenceScalable Cell Culture SystemsViable Cell Count
Abstract This paper describes the design and operation of an industrial type culture system for the submerged growth of BHK 21 cells at a volume of 30 l. Using a modified Eagle's medium and with the culture controlled at pH 7.4 ± 0.05 and at a temperature of 35 ± 0.25°C., the cells grew from 0.5 × 10 6 to about 2.5 × 10 6 viable cells/ml. in 50 hr. at stirrer speeds of 330 and 460 rpm. The significance of certain aspects of design and operation of a culture system of this type is discussed.
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